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抗癌药物阿霉素(ADM)分子量543,为半抗原,制备其特异性抗体难度较大,尚未见文献报道,现将其制备方法、抗体效价测定等简报如下。1 材料和方法 用牛血清白蛋白(BSA)为载体,参照Geffard[Brain Research 1984;294:161～165]、Hurwitz[Cancer Research 1975;35:1175～1181]和黄威权等[第四军医大学学报 1986;7(4):313]的方法制备出 ADM-BSA 结合物,以此结合物为抗原采用多点皮下注射的方法,免疫体重在 2.5 kg 左右的雄性新西兰大白兔,每只动物1次注射1 ml不
The anticancer drug adriamycin (ADM) molecular weight of 543, as a hapten, the preparation of its specific antibodies more difficult, has not been reported in the literature, now its preparation, antibody titer and other briefings are as follows. 1 Materials and Methods Bovine serum albumin (BSA) was used as a vector and Geffard [Brain Research 1984; 294: 161-165], Hurwitz [Cancer Research 1975; 35: 1175-1181] and Huang Weiquan [Fourth Military Medical University (1986; 7 (4): 313], a conjugate of ADM-BSA was prepared. The conjugate was used as a multi-subcutaneous injection of antigens to immunize New Zealand white rabbits weighing about 2.5 kg per animal 1 Injections 1 ml no