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目的研究杏仁对吸烟致DNA氧化损伤和脂质过氧化的保护作用。方法选取60名年龄在18～25岁男性吸烟志愿者,随机分为2组,每组30人,分别先后交叉给予安慰剂(100 g猪肉,相当于84 g杏仁所提供的热量)和84 g杏仁,均持续4周。分别通过HPLC法测定24 h尿样中8-羟基脱氧鸟苷(8-OHdG)和丙二醛(MDA)水平,进行彗星实验测定淋巴细胞DNA链的断裂情况。结果给吸烟者补充杏仁后,尿8-OHdG、MDA和淋巴细胞DNA的断裂分别降低了28%、23%和34%,血清维生素E升高了10%。结论杏仁能有效降低吸烟引起的DNA氧化损伤和脂质过氧化。
Objective To study the protective effects of almonds on DNA oxidative damage and lipid peroxidation induced by smoking. Methods Sixty male smoking volunteers aged 18-25 years old were randomly divided into 2 groups of 30 patients. Each group was crossed with placebo (100 g pork, equivalent to calories provided by 84 g almonds) and 84 g Almonds, all lasting 4 weeks. The 8-hydroxydeoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels in 24 h urine samples were determined by HPLC, and the DNA strand breaks in lymphocytes were measured by comet assay. Results After almonds were added to smokers, DNA 8-OHdG, MDA and lymphocyte DNA were reduced by 28%, 23% and 34%, respectively, and serum vitamin E by 10%. Conclusion Almonds can effectively reduce DNA oxidative damage and lipid peroxidation caused by smoking.