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目的：为了解胆道梗阻后患者血液中内毒素(Endotoxin,ETX)、内皮素( Endothelin,ET)、心钠素(Atrial Natriunetic Peptide,ANP)含量变化及其对肾脏排泄功能的影响。方法：挑选体重为200~250g的雄性Wistar大鼠60只，随机分为3组，每组20只。A组戊巴比妥腹腔麻醉下剖腹缝扎胆总管，B组同法麻醉剖腹不结扎胆管，C组同法麻醉不剖腹。用放射免疫法测定各组术前、术后3d，术后1周、2周、3周血浆ET、ANP含量，用鲎试剂法测定同期ETX，常规生化法测定尿肌酐清除率、尿钠含量，对比不同组、不同时间组内及组间差别，并作相关分析。结果：胆道梗阻动物血浆ETX、ET、ANP明显高于非胆道梗阻者，尿肌酐清除率、尿钠含量明显低于非胆道梗阻者，ETN、ET、ANP随梗阻时间延长而逐渐升高，与胆红素量(TB)呈正相关，尿肌酐清除率、尿钠含量随梗阻时间延长而降低，与TB、ETX、ET、ANP呈负相关、ETX与ET、ANP亦呈明显正相关。结论：胆道梗阻可致患者ETX、ET含量升高，进而导致肾排泄功能下降，这种变化的结果有可能导致肾衰的发生。
Objective: To investigate the changes of endotoxin (ETX), endothelin (ET) and Atrial Natriunetic Peptide (ANP) in the blood of patients with biliary obstruction and their effects on renal excretion. Methods: Sixty male Wistar rats with a body weight of 200 ~ 250g were selected and randomly divided into three groups of 20 rats. A group of pentobarbital anesthesia under abdominal anesthesia with common bile duct, B group anesthesia with cesarean section without ligating bile duct, C group with the same anesthesia is not laparotomy. The levels of plasma ET and ANP in preoperative and postoperative 3d, postoperative 1 week, postoperative 2 weeks and postoperative 3 weeks were determined by radioimmunoassay. ETX was measured by 鲎 reagent method. The urinary creatinine clearance rate and urinary sodium content , Comparing different groups, different time groups and between groups differences, and make correlation analysis. Results: The plasma levels of ETX, ET and ANP in biliary obstructive animals were significantly higher than those in nonbiliary obstructions. Urinary creatinine clearance and urinary sodium excretion were significantly lower than those without biliary obstruction. The levels of ETN, ET and ANP gradually increased with prolonged obstruction, Bilirubin (TB) was positively correlated. Urinary creatinine clearance and urinary sodium decreased with prolonged obstruction, negatively correlated with TB, ETX, ET and ANP. ETX and ET and ANP also showed a significant positive correlation. Conclusion: Biliary tract obstruction can cause ETX, ET levels increased, leading to decreased renal excretion, the result of this change may lead to the occurrence of renal failure.