,A rapid and convenient dot-immunobinding assay for chicken egg-yolk antibodies

• 作 者: GUANG PING RUAN LI MA XIN SHENG YAO QIAN WEN HONG YUN ZOU WEI LUO XIAO NING WANG
• 机 构: Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou,P
• 出 处: 中华微生物学和免疫学杂志（英文版）
• 发表日期: 2006年2期
• 关键词: Egg yolk antibody IgY Dot-immunobinding assay

The dot-immunobinding assay was applied to investigate the characteristics of chicken egg yolk antibodies. This method of assay was proved to be a rapid and simple method to demonstrate and characterize the egg-yolk antibody IgY in comparison with the traditional ELISA assay. By using the BandScan software, the gray scale value of dots and the background could be determined. According to the intensity of dots (gray scale value) compared to the standard sample of 10 μg, how much IgY remained can be determined in a shorter time.