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目的探讨P33ING1在肛管癌中的表达及与P53和细胞凋亡的关系。方法应用免疫组织化学SP法检测42例肛管癌标本中P33ING1和P53的表达,同时检测其细胞凋亡,并与肛管腺瘤和乳头状瘤及肛周炎性肿块对照组比较。结果在肛管癌、肛管腺瘤及乳头状瘤、肛周炎性肿块组织中P33ING1阳性表达率分别为40．5%、97．2%及97．5%；P53分别为50．O%、22．2%及27．5%；平均细胞凋亡指数则分别为(10．27±1．23)‰、(42．67±1．04)‰及(42．75±0．98)‰。肛管癌中P33ING1、P53阳性率及平均细胞凋亡指数与另外两组比较,差异有统计学意义(P＜0．05)。在P53表达阳性的21例病例中有18例P33ING1表达阴性。结论P33ING1在肛管癌组织中低表达,对肛管癌的发生、发展可能起重要作用。P33ING1和P53互相协同,抑制细胞生长、促进调亡。
Objective To investigate the expression of P33ING1 in anal canal cancer and its relationship with P53 and apoptosis. Methods The expression of P33ING1 and P53 in 42 specimens of anal canal cancer was detected by immunohistochemical SP method. The apoptosis of the specimens was compared with that of anal adenoma, papilloma and perianal inflammatory mass. Results The positive expression rates of P33ING1 in anal cancer, anal adenoma and papilloma and perianal inflammatory mass were 40.5%, 97.2% and 97.5%, respectively; P53 was 50%. The average apoptotic index was (10.27 ± 1.23) ‰, (42.67 ± 1.04) ‰ and (42.75 ± 0. 98) ‰. The positive rate of P33ING1, P53 and the average apoptosis index in anal canal cancer were significantly different from the other two groups (P <0.05). Among the 21 cases with positive P53 expression, 18 cases had negative P33ING1 expression. Conclusion The low expression of P33ING1 in anal cancer tissue may play an important role in the occurrence and development of anal cancer. P33ING1 and P53 cooperate with each other to inhibit cell growth and promote apoptosis.