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研究包括380个ICSI周期,ICSI后14～17小时检查原核,形态学参数包括核仁前体的数量(NPB)及其在每个原核的分布(有极性或无极性),具有下列特征的被认为是“O”型受精卵;①2个原核的NPB数量相差不超过3个;②2个原核中的NPB分布(随机或有极性)是相同的;③每个原核至少有3个NPB。在观察中需要调整焦点直到观察到所有原核,观察前要转动受精卵,保证能清楚地看到2个原核,原核的重叠会阻碍观察NPB的数目和分布。ICSI后2天进行胚胎移植,胚胎的选择按照传统标准即卵裂速度和形态,上述的观察结果不被考虑,优质胚胎标准为细胞大小均匀一致,细胞碎片<10％,细胞胞质清亮、无颗粒。超声下宫腔内可见胎囊并有胎心跳动被认为临床妊娠。
The study included 380 cycles of ICSI and 14 to 17 hours after ICSI for prokaryotic morphological parameters including the number of nucleolar precursors (NPB) and their distribution in each pronucleus (polar or non-polar) with the following characteristics Is considered “O” type fertilized eggs; ① 2 prokaryotic NPB number does not differ more than 3; ② 2 prokaryotic NPB distribution (random or polar) are the same; ③ each prokaryotic at least 3 NPB. During the observation, the focus needs to be adjusted until all prokaryotes are observed. Before the observation, the zygotes should be rotated to ensure that two prokaryotes can be clearly seen. The prokaryotic overlap will obstruct the observation of the number and distribution of NPB. Embryo transfer was performed 2 days after ICSI. The selection of embryos was based on the traditional criteria of cleavage rate and morphology. The above observations were not considered. The standard embryo quality was uniform cell size <10%, cell cytoplasm was clear, and no Granules. Ultrasound intrauterine visible fetal sac and fetal heart rate be considered as clinical pregnancy.