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目的定量研究IL-11对中子照射后小鼠肠道损伤的保护作用。方法4.0 Gy中子全身照射136只BALB/C二级小鼠,于照前和照后注射600μg/kg的rhIL-11(每日1次,连用3 d),并于照后6 h、1 d、2 d和5 d活杀取材,采用光镜和图像分析技术研究IL-11对中子照射后肠道病理形态、肠绒毛高度和隐窝细胞数的影响。结果4.0 Gy中子照射后,肠隐窝细胞核肿胀、浓缩及核碎片形成,再生微弱;照前和照后给予IL-11,肠绒毛高度增加、绒毛上皮和隐窝数量增多,有较多新生的肠隐窝,与单纯照射组比较,差异显著(p<0.05或0.01)。结论应用IL-11可减轻中子辐射肠道损伤的程度,增加存活隐窝细胞数量和绒毛高度,并促进其再生,有利于肠粘膜恢复。
Objective To quantitatively study the protective effect of IL-11 on intestinal injury induced by neutron irradiation in mice. Methods A total of 136 BALB / C mice were irradiated with 4.0 Gy neutron and injected with 600 μg / kg rhIL-11 (once daily for 3 days) before and after irradiation. After 6 h, d, 2 d and 5 d. The effects of IL-11 on the pathological changes of intestinal tract, the height of villus and the number of crypt cells after neutron irradiation were studied by light microscope and image analysis. Results After 4.0 Gy neutron irradiation, the intestine crypt nucleus swelled, concentrated and formed nuclear fragments with weak regeneration. IL-11 was administered before and after irradiation, and the villus height increased, the number of villus epithelium and crypts increased, and there were more freshmen (P <0.05 or 0.01), which was significantly different from that of the simple irradiation group. Conclusion The application of IL-11 can reduce the extent of neutron radiation gut injury, increase the number of surviving crypt cells and villus height, and promote their regeneration, which is good for the recovery of intestinal mucosa.