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目的:建立HPLC-RID同时测定咽立爽口含滴丸中樟脑、石竹烯、龙脑、异龙脑的含量的方法。方法:采用依利特C18柱(150mm×4.6mm,5μm);流动相:甲醇-0.4%磷酸溶液(65∶35);流速:1.0m L/min;柱温:40℃;示差检测器。结果:樟脑、石竹烯、龙脑、异龙脑分别在0.102-1.535、0.009-0.162、0.103-1.554、0.107-1.607mg/m L内呈良好的线性关系,回归方程分别为Y=79 284X+4 796.6(r=0.9993)、Y=48 979X-22.275(r=0.9992)、Y=79 415X+2 756.0(r=0.9991)、Y=77 727X+2 635.7(r=0.9990)。平均加样回收率(n=6)分别为101.19%(RSD=1.75%)、101.45%(RSD=1.42%)、100.02%(RSD=0.13%)、99.74%(RSD=1.21%)。结论:该方法简便、准确、重现性好,为以艾片、艾纳香油为主药的中成药的质量控制提供另一种测定方法。
OBJECTIVE: To establish a HPLC-RID method for the simultaneous determination of camphor, caryophyllene, borneol and isoborneol in the pills of pharynx and refreshing drops. Methods: Ellite C18 column (150mm × 4.6mm, 5μm) was used. The mobile phase was methanol-0.4% phosphoric acid solution (65:35). The flow rate was 1.0m L / min. Results: There was a good linear relationship between camphor, caryophyllene, borneol and isoborneol in the range of 0.102-1.535,0.009-0.162,0.103-1.554,0.107-1.607mg / m L, the regression equation was Y = 79284X + 4 796.6 (r = 0.9993), Y = 48 979X-22.275 (r = 0.9992), Y = 79 415X + 2 756.0 (r = 0.9991), Y = 77 727X + 2 635.7 (r = 0.9990). The average recoveries (n = 6) were 101.19% (RSD = 1.75%), 101.45% (RSD = 1.42%), 100.02% (RSD = 0.13%) and 99.74% (RSD = 1.21%), respectively. Conclusion: The method is simple, accurate and reproducible. It provides an alternative method for the quality control of proprietary Chinese medicines based on radix etoposide and.