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Objective:To investigate the function of cytokines,chemokines,and regulatory T cells(Tregs)in the pathogenesis of Type 1 diabeles mellitus(T1DM)in children.Methods:A total of 35 children with T1DM and 30 healthy controls were enrolled in this study.Levels of serum cytokines(IL-1α,IL-6,IL-10,IL-12,and TNF-α)and chemokines(MIP-1α,MIP-1βand MCP-1)were detected by enzyme-linked immunosorbent assay.Peripheral blood mononuclear cells(PBMCs)were isolated and culture supernatant of phytohaemagglutinin(PHA)-stimulatcd PBMCs was subjecled to ELISA for levels of cytokines(IL-1α,IL-6,IL-10,IL-12 and TNV-α)in T1DM and control group.Furthermore,flow cytometty was used to determine the percentage of Tregs in PBMCs of two groups.Results:Levels of serum cytokines including IL-1α,IL-6,IL-10 andd TNF-αas well as chemokines,such as MIP-1αand MIP-1βin children with T1DM children were significantly higher than those in healthy controls(P<0.05,respectively).PBMCs with PHA stimulation in T1DM group secreted more IL-1αand TNF-α(P<0.05,respectively),but less IL-10(P<0.05),as compared with control group.Furthermore,the proportion of CD4~+,CD25~+,Foxp3.Tregs in PBMCs isolated from children with T1DM was obviously lower than those in heathy controls(P<0.05).Conclusions:Immune dysfunction.with uprcgulation of inflanunatory factors such as IL-1α.IL-6.TNF-αand MIP-1α.downregulation of IL-10 and Tregs,plays an important role in the pathogenesis of T1DM in children.
Objective: To investigate the function of cytokines, chemokines, and regulatory T cells (Tregs) in the pathogenesis of Type 1 diabeles mellitus (T1DM) in children. Methods: A total of 35 children with T1DM and 30 healthy controls were enrolled in this study .Levels of serum cytokines were detected by enzyme-linked immunosorbent assay (IL-1α, IL-6, IL-10, IL- 12, and TNF- α) and chemokines (MIP- 1α, MIP- 1βand MCP- 1) Peripheral blood mononuclear cells (PBMCs) were isolated and culture supernatant of phytohaemagglutinin (PHA) -stimulatcd PBMCs were subjecled to ELISA for levels of cytokines (IL-1α, IL-6, IL-10, IL-12 and TNV-α) T1DM and control group. Hotmore, flow cytometty was used to determine the percentage of Tregs in PBMCs of two groups. Results: Levels of serum cytokines including IL-1α, IL-6, IL-10 and TNF-α as well as chemokines, such as MIP-1αand MIP-1βin children with significantly higher than those in healthy controls (P <0.05, respectively). PBMCs with PHA stimulation in T1DM group The proportion of CD4 ~ +, CD25 ~ +, Foxp3.regs in (P <0.05, respectively), but less IL-10 PBMCs isolated from children with T1DM was obviously lower than those in heathy controls (P <0.05) .Conclusions: Immune dysfunction. With uprculation of inflanunatory factors such as IL-1α.IL-6.TNF-αand MIP-1α. Downregulation of IL -10 and Tregs, plays an important role in the pathogenesis of T1DM in children.