论文部分内容阅读
目的：分析两种梅毒检测方法的临床意义。方法：应用酶联免疫吸附试验（ELISA）和梅毒甲苯胺红不加热血清试验(TRUSE)对北京市某医院2015年6月~9月间对新入所的1247例人员进行的抗TP抗体检测。比较分析ELISA法与TRUSE法检测的结果。结果：1247例标本中ELISA阳性病例86例，感染率6.89%；胶体金法阳性病例61例,感染率4.89%；TURSE法阳性病例27，感染率2.16%。以胶体金法结果为金标准，对TRUSE法与ELISA法检测梅毒抗体的结果进行临床诊断性试验的比较，TURSE法检测阳性率低于ELISA（P<0.05）。结论：TURSE法检测灵敏度比ELISA低，并且受人为因素影响较大，不适用于梅毒螺旋体感染初筛的检测。ELISA检测梅毒螺旋体抗体的敏感性和特异性较高，适用与临床大样本量筛查。“,”Objective: to analyze the clinical significance of the detection methods of two kinds of syphilis. Methods: using the enzyme-linked immune adsorption assay (ELISA) and Syphilis toluidine red unheated serum test (TRUSE) detection in a hospital in Beijing by 2015 from 6 months to 9 months to new into the 1247 cases of the anti TP antibody detection. Comparative analysis of the results of ELISA reagents and coloidal gold detection. Results: 1247 cases of ELISA positive cases in 86 cases, the infection rate was 6.89%; 61 cases of coloid gold positive cases, the infection rate was 4.89%; TRUSE positive cases 27, the infection rate was 2.16%. With coloidal gold method as a gold standard, the results of TRUSE method and ELISA method for the detection of syphilis antibody clinical diagnostic tests are compared.The positive rate of TRUSE was lower than that of ELISA ( P<0.05). . Conclusion: TRUSE assay sensitivity than ELISA is low, and by human factors influence is big, is not applicable in the early infection of Treponema palidum sieve detection. The sensitivity and specificity of ELISA for the detection of syphilis antibody were higher, and the clinical application of large sample size screening.