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目的:建立木香槟榔丸中木香烃内酯和去氢木香内酯的含量测定方法。方法:采用HPLC,色谱柱为Agilent C18(4.6 mm×250 mm,5μm),流动相甲醇-水(65∶35),检测波长225 nm,柱温30℃,流速1.0 mL.min-1。结果:木香烃内酯和去氢木香内酯的线性范围分别为0.046 4～0.3 712μg(r=0.999 6)和0.045 2～0.361 6μg(r=0.999 5);平均加样回收率分别为99.0%(RSD 0.86%,n=6)和99.2%(RSD 0.23%,n=6)。结论:该法简便,快速,结果准确,重复性好,可用于控制木香槟榔丸的质量。
OBJECTIVE: To establish a method for the determination of caromocarbene and dehydrocostuslane in betel nut tablets. Methods: The mobile phase was Agilent C18 (4.6 mm × 250 mm, 5 μm) with mobile phase of methanol-water (65:35) at a detection wavelength of 225 nm. The column temperature was 30 ℃ and the flow rate was 1.0 mL · min-1. Results: The linear ranges of the wood and lactone were 0.046 4 ~ 0.3 712 μg (r = 0.999 6) and 0.045 2 ~ 0.361 6 μg (r = 0.999 5) respectively. The average recoveries were 99.0% (RSD 0.86%, n = 6) and 99.2% (RSD 0.23%, n = 6). Conclusion: The method is simple, rapid, accurate, reproducible and can be used to control the quality of betel nut tablets.