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目的:探讨肾脏和尿液AQP2的表达与糖尿病肾病(DN)大鼠水液代谢异常关系及泽黄颗粒防治DN效应机制。方法:用高脂高糖饲料加小剂量STZ诱导DM大鼠模型,随机分为糖尿病模型组(DM组)、泽黄颗粒治疗组(Z组)和格列吡嗪对照组(G组),正常对照组(N组)。于实验第12周末检测BG、HbA1c、Ccr、UAER及血液流变学指标;观察肾组织病理改变;运用Western-Blot、Real-Time PCR法检测肾脏AQP2蛋白及mRNA表达变化,并检测尿液内AQP2浓度的变化。结果:与DM组相比,Z组大鼠BG、HbA1c、UAER、全血黏度、血浆黏度、肾脏AQP2蛋白、AQP2 mRNA表达和尿液内AQP2浓度均明显下降(P<0.05),Ccr明显升高(P<0.01)。与G组相比,Z组大鼠全血黏度、TG、TC水平明显降低(P<0.05)。结论:泽黄颗粒可下调糖尿病大鼠肾脏和尿液AQP2的表达,这可能是其调节水液代谢的机制和防治DN的基础。
Objective: To investigate the relationship between renal and urinary AQP2 expression and abnormal water-liquid metabolism in diabetic nephropathy (DN) rats and the mechanism of Zehuang granule in preventing and treating DN. Methods: DM rats were induced with high-fat and high-sugar diet and low-dose STZ and were randomly divided into diabetes model group (DM group), Zehuang granule treatment group (Z group) and glipizide control group Normal control group (N group). At the end of the twelfth week of experiment, BG, HbA1c, Ccr, UAER and hemorheology indexes were detected; the pathological changes of kidney were observed; the expression of AQP2 protein and mRNA in kidney were detected by Western-Blot and Real-Time PCR; AQP2 concentration changes. Results: Compared with DM group, the contents of BG, HbA1c, UAER, whole blood viscosity, plasma viscosity, renal AQP2 protein, AQP2 mRNA and urine AQP2 in Z group were significantly decreased (P <0.05) High (P <0.01). Compared with group G, whole blood viscosity, TG and TC in Z group were significantly decreased (P <0.05). Conclusion: Zehuang granule can down-regulate the expression of AQP2 in kidney and urine of diabetic rats, which may be the mechanism of its regulation of aqueous and liquid metabolism and the basis of prevention and treatment of DN.