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目的:研究ZFHX3基因rs7193343多态性与心房颤动(房颤)的相关性。方法:采用前瞻式病例对照研究,按照相关标准入选202例汉族人群,其中房颤患者102例,无房颤患者100例,收集其临床资料和静脉血标本,分别提取基因组DNA,通过聚合酶链反应-限制性片段长度多态性方法和基因测序法检测所有患者的ZFHX3基因rs7193343多态性的基因型。结果:①2组患者ZFHX3基因rs7193343的CC基因型(P=0.023,OR=0.422,95%CI0.198～0.900)、TT基因型(P=0.024,OR=2.475,95%CI1.106～5.541)及等位基因(P=0.017,OR=1.614,95%CI1.089～2.391)的分布均差异有统计学意义。②房颤患者中,TT基因型患者的超敏C反应蛋白水平较CC基因型患者明显高[1.62(0.90～2.90)mg/L∶0.71(0.34～1.09)mg/L,P=0.014],且TT基因型患者的左房前后径和右房横径大小均较CC基因型患者明显增大[(38.2±4.9)mm∶(33.5±4.1)mm,P=0.026;(38.9±6.0)mm∶(33.1±2.6)mm,P=0.003]。结论:在大陆汉族人群中,ZFHX3基因rs7193343多态性与房颤具有显著的相关性,T等位基因和TT基因型是房颤发生发展的危险因子,可通过影响心房肌的结构重构和炎症性改变为房颤的发生、发展提供基质。
Objective: To study the association between rs7193343 polymorphism of ZFHX3 gene and atrial fibrillation (AF). Methods: A prospective case-control study was conducted in 202 Han Chinese subjects according to the relevant criteria, including 102 patients with atrial fibrillation and 100 patients without atrial fibrillation. The clinical data and venous blood samples were collected and genomic DNA was extracted separately. The genotypes of rs7193343 polymorphism of ZFHX3 gene in all patients were detected by response-restriction fragment length polymorphism and gene sequencing. Results: ①The CC genotypes of ZFHX3 rs7193343 in two groups (P = 0.023, OR = 0.422, 95% CI0.198-0.900), TT genotype (P = 0.024, OR = 2.475, 95% CI 1.106-5.541) And alleles (P = 0.017, OR = 1.614, 95% CI1.089-2.391) showed statistically significant differences. ② In patients with atrial fibrillation, the TT genotypes were significantly higher in patients with CC genotype than those with CC genotype [1.62 (0.90-2.90) mg / L: 0.71 (0.34-1.09) mg / L, P = 0.014] Compared with patients with CC genotype, TT genotype patients had significantly increased anteroposterior diameter and right atrial diameter (38.2 ± 4.9) mm: (33.5 ± 4.1) mm, P = 0.026; (38.9 ± 6.0) mm : (33.1 ± 2.6) mm, P = 0.003]. Conclusion: There is a significant correlation between rs7193343 polymorphism of ZFHX3 gene and atrial fibrillation in mainland Han population. T allele and TT genotype are risk factors for the development of atrial fibrillation. Inflammatory changes provide the basis for the development of atrial fibrillation.