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目的建立一种高特异性和灵敏度的卵巢癌造影方法。方法通过活泼酯法将量子点(QDs)与叶酸(FA)耦联。通过透射电子显微镜(TEM)、动态光散射检测(DLS)、琼脂糖凝胶电泳和傅里叶红外光谱(FTIR)对QDs或叶酸耦联的量子点(FA-QDs)进行表征,并与卵巢癌SKOV3细胞共同孵育观察其染色效果。结果本实验制备的FA-QDs性质稳定、大小均一、分散性良好。耦联FA后的QDs在凝胶电泳中移动速度明显减慢,并在FTIR中见新的化学键形成。与对照组比较(孵育QDs后的SKOV3细胞),孵育FA-QDs后的SKOV3细胞在荧光显微镜下清晰可见,并在染色后48 h仍不褪色。结论成功制备了一种特异性好、敏感度高的细胞水平造影剂,在卵巢癌的早期检测中具有潜在的应用价值。
Objective To establish a high specificity and sensitivity of ovarian cancer imaging method. Methods Quantum dots (QDs) were coupled with folic acid (FA) by an activated ester method. QDs or folic acid-coupled quantum dots (FA-QDs) were characterized by transmission electron microscopy (TEM), dynamic light scattering detection (DLS), agarose gel electrophoresis and Fourier transform infrared spectroscopy (FTIR) Cancer SKOV3 cells incubated together to observe the staining effect. Results FA-QDs prepared in this study were stable in nature, uniform in size and good in dispersibility. QDs coupled with FA were significantly slower in gel electrophoresis and new chemical bonds were formed in FTIR. Compared with the control group (SKOV3 cells after QDs incubation), the SKOV3 cells after FA-QDs incubation were clearly visible under a fluorescence microscope and did not fade 48 h after staining. Conclusion A good specificity and high sensitivity of cell-level contrast medium has been successfully prepared and has potential value in the early detection of ovarian cancer.