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脯氨酸是一种组成蛋白质的普通氨基酸。许多快速生长的细胞都有脯氨酸浓度高的特点。脯氨酸的功能不仅直接参与蛋白质的组成,还有促进细胞内一些氧化还原作用。在植株枯萎、营养不足或发生病害的条件下,则会产生脯氨酸的积累。本研究用脯氨酸—14C为标记,测定脯氨酸在大麦幼苗中的分布和转化。结果表明,在2℃低温和25℃高温下发芽的大麦幼苗都能茁壮生长。高温发芽幼苗,其重量和株高都胜于低温发芽的幼苗。而放射性的分布以掺入根部的同位素比掺入地上部的多。高温幼苗中掺入根与苗之间的放射性差异比低温幼苗的大。
Proline is a common amino acid that makes up the protein. Many rapidly growing cells have high concentrations of proline. The function of proline not only directly involved in the composition of the protein, but also promote some redox role within the cell. In plant withered, undernourished or disease conditions, it will produce proline accumulation. In this study, Proline-14C was used as a marker to determine the distribution and transformation of proline in barley seedlings. The results showed that barley seedlings germinated at low temperature of 2 ℃ and high temperature of 25 ℃. High temperature sprouting seedlings, its weight and plant height are better than low temperature sprouting seedlings. The distribution of radioactive isotope incorporation of the root than the incorporation of more than the ground. Radiation differences between roots and shoots in high temperature seedlings were larger than those in low temperature seedlings.