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目的建立荧光衍生化法测定杏干中总亚硫酸盐的方法。方法使用NAM[N-(9-吖啶基)马来酰亚胺]为衍生化试剂,其与亚硫酸盐发生衍生化反应生成强荧光产物,采用荧光法对杏干中的总亚硫酸盐含量进行测定。结果该法的线性范围为1.20 mg/L~43.2 mg/L,线性回归方程为y=38.99x+398.88,相关系数为0.999 3;该方法的最低检出限为0.50μg;回收率为92.2%~100.2%,相对标准偏差(RSD)≤3.80%。结论该方法测定结果与国家标准方法检测结果基本一致,为干果中总亚硫酸盐含量的快速测定提供可选择的方法。
Objective To establish a fluorescence derivatization method for determination of total sulfite in dried apricots. Methods NAM [N- (9-acridinyl) maleimide] was used as a derivatization reagent, which was derivatized with sulfite to generate a strong fluorescent product. The total sulfite content Content was measured. Results The linear range of this method was 1.20 mg / L ~ 43.2 mg / L, the linear regression equation was y = 38.99x + 398.88, the correlation coefficient was 0.999 3. The detection limit of this method was 0.50 μg. The recovery was 92.2% ~ 100.2%, relative standard deviation (RSD) ≤3.80%. Conclusion The results of this method are in good agreement with those of the national standard method. It provides an alternative method for the rapid determination of total sulfite content in dried fruits.