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目的:观察绞股蓝总皂苷(GP)对全脑缺血再灌注大鼠海马、大脑皮层、纹状体及齿状回的保护作用.方法:采用4-血管阻断(4-VO)方法建立大鼠急性全脑缺血模型,用吖啶橙染色法观察不同脑区DNA和RNA含量的变化.结果:与正常对照组比较,全脑缺血再灌注大鼠各脑区DNA和RNA吖啶橙染色后的荧光强度(反映DNA和RNA含量)明显减弱;GP100 mg/kg ig给药组各脑区DNA和RNA吖啶橙染色后的荧光强度强于全脑缺血再灌注模型组,与正常对照组相似.结论:GP明显减轻缺血再灌注对大鼠海马、大脑皮层、纹状体及齿状回DNA和RNA损伤.
Objective: To observe the protective effect of gypenosides (GP) on hippocampus, cerebral cortex, striatum and dentate gyrus in rats with global cerebral ischemia and reperfusion. METHODS: To establish a large size using 4-vascular occlusion (4-VO) method. Acute global cerebral ischemia model in rats was used to observe the change of DNA and RNA content in different brain regions by acridine orange staining. Results: Compared with the normal control group, DNA and RNA of each brain region of rats with cerebral ischemia-reperfusion were recorded. The fluorescence intensity after staining (reflecting DNA and RNA content) was significantly attenuated; the fluorescence intensity of DNA and RNA acridine orange staining in each brain region of GP 100 mg/kg ig group was stronger than that of global cerebral ischemia reperfusion model group, and normal The control group was similar. Conclusion: GP can significantly reduce the damage of DNA and RNA in hippocampus, cerebral cortex, striatum and dentate gyrus of rats after ischemia and reperfusion.