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应用间歇淋洗培养方法 ,以长期不同培肥定位试验土壤为研究对象 ,求得土壤氮素矿化参数 ,并探讨氮素矿化潜势 (N0 )、碱解氮、微生物氮、可浸提易矿化氮、全氮之间的关系。结果表明 ,在 35℃和 2 0℃条件下培养 ,一级动力学模型能够很好的拟合试验数据 ,模拟方程和模拟参数均达到极显著水平。经过 15年的培肥和轮作 ,无论是单施氮肥区 ,还是氮肥与有机肥配合施用区 ,N0 均有不同程度的增加 ,这标志着土壤活性有机氮库增加。k值变化范围在 0 0 0 4 6 2 8～0 0 1314 8d- 1之间 ,说明可矿化氮以每天 0 46 %～ 1 31%的平均速率矿化释放。而且 ,在本试验条件下 ,淋洗液中均含有一定数量的可溶性有机态N ,因此进行氮素矿化研究时 ,同时测定NH4 N、NO3 N和Norg的含量是必要的。 35℃下 ,N0 占全氮的比例为 7 2 3%～ 17 36 % ,变异系数 30 4% ;易矿化有机态氮占全氮的比例为 0 2 7%～ 0 48% ,变异系数 2 0 0 % ;碱解氮占全氮的比例为 5 55%～ 6 54% ,变异系数仅 5 8% ;微生物氮占全氮的比例在 2 16 %～ 5 18%之间 ,变异系数 2 8 8%。从几种指标测得的平均值看 ,N0 >碱解氮 >微生物氮 >易矿化氮 ,而变异系数是N0 >微生物氮 >易矿化氮 >碱解氮。虽然N0 的绝对值远高于田间实际矿化量 ,?
The application of intermittent leaching culture method to long-term different fertilization locating soil test as the object of study to obtain soil nitrogen mineralization parameters, and explore the nitrogen mineralization potential (N0), alkaline hydrolysis of nitrogen, microbial nitrogen, leachable The relationship between easily mineralized nitrogen and total nitrogen. The results showed that the first-order kinetic model could well fit the experimental data when incubated at 35 ℃ and 20 ℃, and the simulated and simulated parameters reached the extremely significant level. After 15 years of fertilizing and rotation, both N0 and N0 increased with the increase of soil active N accumulation, either in the single application of nitrogenous fertilizer or in combination with the application of nitrogen fertilizer and organic fertilizer. The value of k varies from 0 0 0 4 6 2 8 to 0 0 1314 8d-1, indicating that mineralizable nitrogen is mineralized at an average rate of 0 46% to 1 31% per day. Moreover, under the experimental conditions, the eluent contains a certain amount of soluble organic N, therefore it is necessary to simultaneously determine the contents of NH4N, NO3N and Norg during nitrogen mineralization. The ratio of N0 to total nitrogen was 7 2 3% to 17 36% and the coefficient of variation was 30 4% at 35 ℃. The ratio of readily mineralizable organic nitrogen to total nitrogen was 0 2 7% ~ 0 48%, and the coefficient of variation 2 0 0%; the ratio of total nitrogen to total nitrogen was 555% ~ 544%, the coefficient of variation was only 58%; the proportion of microbial nitrogen to total nitrogen was between 2 16% ~ 5 18%, the coefficient of variation was 2 8 8%. According to the average values measured by several indexes, N0> alkaline hydrolysis nitrogen> microbial nitrogen> readily mineralizable nitrogen, and the coefficient of variation is N0> microbial nitrogen> easily mineralizable nitrogen> alkaline hydrolysis nitrogen. Although the absolute value of N0 is much higher than the actual mineralization in the field,?