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目的:探讨RNA编辑酶1(RNA-dependent adenosine deam inase1,ADAR1)mRNA在喉癌及癌旁组织的表达及意义。方法:采用半定量RT-PCR分别检测51例喉癌患者的癌及癌旁组织ADAR1 mRNA的表达。结果:51例喉癌及其癌旁组织ADAR1 mRNA均有表达,相对表达量分别为2.963±0.912、0.791±0.197,两者间差异有统计学意义(P<0.01);对照组27例ADAR1 mRNA的相对表达量为0.910±0.311,与喉癌组织比较差异有统计学意义(P<0.01),与癌旁组织比较差异无统计学意义(P>0.05)。将喉癌组织和癌旁组织A-DAR1 mRNA表达量进行配对t检验,差异有统计学意义(P<0.01)。结论:ADAR1 mRNA在喉癌组织中表达增高,可能在喉癌的发生机制中起一定的作用。
Objective: To investigate the expression and significance of RNA-dependent adenosine deam inase1 (ADAR1) mRNA in laryngeal carcinoma and its adjacent tissues. Methods: Semi-quantitative RT-PCR was used to detect the expression of ADAR1 mRNA in 51 cases of laryngeal carcinoma and adjacent tissues. Results: The expression of ADAR1 mRNA in 51 cases of laryngeal squamous cell carcinoma and adjacent tissues was 2.963 ± 0.912 and 0.791 ± 0.197, respectively, with significant difference (P <0.01). In the control group, ADAR1 mRNA The relative expression level was 0.910 ± 0.311, which was significantly different from that of laryngeal carcinoma (P <0.01). There was no significant difference between the two groups (P> 0.05). The expression of A-DAR1 mRNA in laryngeal carcinoma and paracancerous tissue was paired t test, the difference was statistically significant (P <0.01). Conclusion: The increased expression of ADAR1 mRNA in laryngeal carcinoma may play a role in the pathogenesis of laryngeal carcinoma.