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[目的]探讨他莫昔芬联合丝裂霉素对HepG2细胞株中ERβ表达、细胞生长及凋亡等影响。[方法]实验分他莫昔芬组、丝裂霉素组、两药联合组及对照组4组,MTT法、流式细胞仪检测他莫昔芬、丝裂霉素对HepG2细胞的生长抑制和细胞凋亡作用,免疫组化SP法及WesternBlot法检测上述4组作用后肝癌细胞ERβ蛋白的表达情况。[结果]与对照组相比,他莫昔芬、丝裂霉素及两药联合组均能抑制细胞生长,并且所有加药组均可诱导细胞凋亡,且联合组效果更明显。他莫昔芬、丝裂霉素及两药联合组均可下调ERβ的表达,两药联合组ERβ表达与丝裂霉素组、他莫昔芬组相比更低。[结论]他莫昔芬可协同丝裂霉素抑制肝癌HepG2细胞生长、诱导其凋亡,该作用可能与其下调HepG2细胞中ERβ的表达相关。
[Objective] To investigate the effects of tamoxifen combined with mitomycin on the expression of ERβ, cell growth and apoptosis in HepG2 cells. [Methods] The tamoxifen group, mitomycin group, two drug combination group and control group were divided into 4 groups. MTT assay and flow cytometry were used to detect the growth inhibition of HepG2 cells by tamoxifen and mitomycin And the role of apoptosis, immunohistochemical SP method and Western Blot detection of the above four groups of liver cancer cells ERβ protein expression. [Result] Compared with the control group, tamoxifen, mitomycin and the combination of the two drugs could inhibit the cell growth, and all the dosing groups could induce apoptosis, and the combined effect was more obvious. Tamoxifen, mitomycin and the combination of the two drugs can reduce the expression of ERβ, ERβ expression in the combination of two groups compared with mitomycin group, tamoxifen group was lower. [Conclusion] Tamoxifen can inhibit mitochondrial growth and induce apoptosis of hepatocellular carcinoma HepG2 cells, which may be related to its downregulation of ERβ expression in HepG2 cells.