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发光二极管诱导荧光检测器(LED-IF)是近十年来发展起来的一种微型化荧光检测器,在流动注射、毛细管液相色谱、毛细管电泳及芯片电泳等微流动分析系统中具有广泛的应用。本文讨论了LED-IF的4种光学结构,并对其主要器件,包括光源、滤光片、透镜、光纤、光电检测器以及检测池作了详细讨论,还介绍了LED-IF与其它技术的联用及其在生物、医药和环境样品检测中的应用,对未来的发展趋势作了展望。
LED-IF (Fluorescent Induced Fluorescence Detector) is a miniature fluorescence detector developed in recent ten years. It has a wide range of applications in micro-flow analysis systems such as flow injection, capillary liquid chromatography, capillary electrophoresis and chip electrophoresis . This article discusses the four optical architectures of the LED-IF and discusses in detail the major components of the LED-IF, including light sources, filters, lenses, optical fibers, photodetectors, and test cells. It also introduces LED-IF and other technologies Combined with its application in the biological, pharmaceutical and environmental samples testing, the future development trend is forecasted.