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为了了解丝虫感染不同感染期的淋巴细胞免疫反应,作者在孟买附近的丝虫病流行区观察了37名受检者,详细记载了他们的年龄,居住在流行区的时间,病史和体检结果,夜间涂血片检查,并作微丝蚴计数。将受检组分成三组,第1组12例为无任何丝虫感染症状的正常人,第2组9例为曾有微丝蚴血症的丝虫感染者,检查时发现其中经海群生治疗的5例(2a组)中4例无微丝蚴(mf),1例仍有9mf/20μl,未经治疗的4例(2b组)微丝蚴密度为1—4mf/20μl,第3组为16例四肢象皮肿病人。另设对照组9人,远离流行区而很少有感染的机会。取班氏丝虫病病人的微丝蚴作抗原,夜间取血经肝素处理,核孔薄膜过滤,冲洗,然后将mf混悬在pH7.4的PBS中;彭亨丝虫微丝蚴取自感染的多乳鼠的血,经Percoll梯度分离后混悬于PBS中,成虫也取自上述动物,洗净后制成匀浆。以上三种抗原再经
To understand the lymphocyte immune response at various stages of infection with filarial infections, the authors observed 37 subjects in the endemic area of filariasis near Mumbai, detailing their age, time of residence in the endemic area, medical history and physical examination results , Smear night inspection, and microfilaria count. The test group was divided into three groups, the first group of 12 cases without any symptoms of filariasis of the normal people, the second group of 9 cases of microfilariae filamentous filariasis who had checked, found that the sea by students Among the 5 cases treated (group 2a), there were 4 cases without microfilaria (mf), 1 case with 9mf / 20μl, 4 cases without treatment (Group 2b) with microfilament density of 1-4mf / 20μl and 3 Group of 16 limb edema patients. Another set of 9 control group, away from the epidemic area and rarely infected with the opportunity. Fetillary filariasis patients taking microfilariae as antigen, blood was taken at night by heparin treatment, nuclear membrane filtration, washing, and then mf suspended in PBS pH7.4; Paenibacillary microfilariae taken from infected The blood of many suckling mice was separated by Percoll gradient and suspended in PBS. Adults were also taken from the above animals and washed to make homogenates. More than three antigens by