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目的探讨ATP敏感型钾离子通道的活性状态对鞘内注射腺苷类似物R-phenylisopropyladenosine(R-PIA)抗伤害性作用的影响。方法雄性SD大鼠在苯巴比妥钠麻醉下,蛛网膜下腔留置PE-10导管,测定注药后鼠尾对光热刺激的反应(抗伤害作用)。结果蛛网膜下腔注射R-PIA产生剂量依赖性的甩尾时间曲线上移(P<0.05)。蛛网膜下腔注药后10min起效,镇痛时间长达60min。ATP敏感型通道开放剂nicorandil和阻滞剂glibenclamide单独蛛网膜下腔应用无镇痛作用(P>0.05),但nicorandil明显增强R-PIA的抗伤害性作用,相反glibenclamide明显减弱R-PIA的抗伤害性作用(P<0.05)。结论蛛网膜下腔注射R-PIA可产生明显的剂量依赖性抗伤害作用,此作用受ATP敏感型钾离子通道活性调节。
Objective To investigate the effect of ATP-sensitive K + channels on the antinociceptive effects of intrathecal adenosine analogue R-phenylisopropyladenosine (R-PIA). Methods Male Sprague-Dawley rats were anesthetized with phenobarbital and the PE-10 catheter was placed in the subarachnoid space to determine the response of the tail to photothermal stimulation (anti-injury). Results The subarachnoid injection of R-PIA produced a dose-dependent tailgating time curve shift (P <0.05). Subarachnoid injection 10min onset after injection, analgesic time up to 60min. Nicorandil, an ATP-sensitive channel opener, and glibenclamide alone had no analgesic effect (P> 0.05), but nicorandil significantly enhanced the anti-nociceptive effect of R-PIA, whereas glibenclamide significantly attenuated R-PIA Nociceptive effect (P <0.05). Conclusion Subarachnoid injection of R-PIA can produce a significant dose-dependent anti-injury effect, which is regulated by ATP-sensitive potassium channel activity.