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目的:研究11-羰基-β-乳香酸的抗癌作用,并探讨其作用机制。方法:用MTT法和生长曲线法检测11-羰基-β-乳香酸对人结肠癌HCT-116细胞增殖的抑制作用,计算半数有效抑制浓度(IC50)值;用Hoechst33258荧光染色法观察经药物处理后细胞核的形态变化;用流式细胞仪测定药物处理后HCT-116的细胞周期和细胞凋亡率。结果:结果提示11-羰基-β-乳香酸浓度为15～50μg/ml时,对HCT-116细胞的48h抑制率为8.7%～90.16%,48h的IC50为27.36±0.14μg/ml;生长曲线结果提示11-羰基-β-乳香酸对HCT-116细胞生长的抑制作用呈明显的时效和量效关系;30μg/ml的药物作用48h后,Hoechst33258染色结果显示细胞核出现细胞核固缩,染色质不均一或边集呈致密强荧光,凋亡小体等明显的凋亡形态学变化;流式仪检测结果提示11-羰基-β-乳香酸可阻滞细胞周期并诱导细胞凋亡。结论:11-羰基-β-乳香酸在体外对结肠癌具有较强的抗癌作用,能够阻断HCT-116细胞周期,诱导细胞凋亡。
Objective: To study the anticancer effect of 11-carbonyl-β-boswellic acid and its mechanism of action. Methods: The inhibitory effect of 11-carbonyl-β-boswellic acid on the proliferation of human colon cancer HCT-116 cells was detected by MTT assay and growth curve method, and the half effective inhibitory concentration (IC50) was calculated. Fluorescent staining with Hoechst33258 After the morphological changes of the nucleus, the cell cycle and apoptosis rate of HCT-116 cells after drug treatment were determined by flow cytometry. Results: The results showed that the 48h inhibition rate of HCT-116 cells was 8.7% -90.16% when the concentration of 11-carbonyl-β-boswellic acid was 15-50μg / ml, and the IC50 was 27.36 ± 0.14μg / ml at 48h. The results showed that the inhibitory effect of 11-carbonyl-β-boswellic acid on the growth of HCT-116 cells was significantly aging and dose-effect relationship; Hoechst33258 staining results showed nuclear pyknosis, chromatin was not observed after 30μg / ml of drug treatment for 48h The results of flow cytometry indicated that 11-carbonyl-β-boswellic acid could block the cell cycle and induce apoptosis. CONCLUSION: 11-carbonyl-β-boswellic acid has a strong anticancer effect on colon cancer in vitro, which can block the cell cycle of HCT-116 cells and induce apoptosis.