论文部分内容阅读
运用PCR技术对皱纹盘鲍(Haliotis discus hannai)、黑足鲍(H.iris)和黑唇鲍(H.rubra)3种鲍16S rRNA基因扩增,纯化后测序。结果表明:皱纹盘鲍、黑足鲍和黑唇鲍序列长度分别为544、546、550 bp,序列AT含量分别为58.1%、56.1%和55.9%,三者序列同源性高达94.18%,碱基变异位点分别集中在9~34 bp、248~295 bp、326~375 bp。所得结果与Genebank中22种鲍相应序列进行对比分析,25种鲍遗传距离距离在0.00~0.14之间;用NJ和ML法绘制系统发育树分析其亲缘关系,结果表明鲍科系统发育呈现出一定的区域性,并且染色体数目相同的物种容易聚为一支。
The 16S rRNA gene of three species of Haliotis discus hannai, H. iris and H. rubra was amplified by PCR and sequenced. The results showed that the length of sequences of Abalone Haliotis discus hannai, Abalone abalone and Abalone abalone were 544,546 and 550 bp, respectively. The AT contents of the sequences were 58.1%, 56.1% and 55.9%, respectively. The homologies of the three sequences were as high as 94.18% The base mutation sites were 9 ~ 34 bp, 248 ~ 295 bp and 326 ~ 375 bp, respectively. The results were compared with the corresponding sequences of 22 species in Genebank. The genetic distance of 25 species was between 0.00 and 0.14. Phylogenetic tree was used to analyze the phylogenetic relationship between NJ and ML. Of the regional, and the number of chromosomes the same species easily get together.