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目的研究新型微生物杀菌制剂NBF毒性,为其使用安全性提供实验依据。方法对微生物杀菌制剂NBF进行小鼠急性经口毒性试验、大鼠急性经皮毒性试验、小鼠骨髓嗜多染红细胞微核试验、家兔多次完整皮肤刺激试验、急性眼刺激试验、豚鼠皮肤变态反应试验、鱼类延长毒性14d试验、螺的生存试验。结果微生物杀菌制剂NBF对小鼠急性经口毒性LD50﹥5000mg/kg·bw,属于实际无毒级物质;大鼠急性经皮毒性LD50﹥2000mg/kg·bw,属低毒级物质;小鼠骨髓嗜多染红细胞微核试验该杀菌制剂各剂量组与阴性对照组比较,微核率差异无统计学意义(P﹥0.05);对家兔多次完整皮肤刺激反应积分为0,急性眼刺激积分指数为0,均属无刺激性;对豚鼠皮肤变态反应试验试验组动物与阴性对照组动物无可见不同,试验组动物皮肤致敏反应积分为0,无致敏作用;鱼类延长毒性14d试验及螺的生存试验均无异常。结论在本实验条件下,未发现微生物杀菌制剂NBF有明显的毒性作用。
OBJECTIVE To study the toxicity of NBF, a novel microbicidal preparation, to provide experimental evidence for the safety of its use. Methods Acute oral toxicity test, rat acute dermal toxicity test, mouse bone marrow polychromatic erythrocyte micronucleus test, rabbit multiple complete dermal irritation test, acute eye irritation test, guinea pig skin Allergy test, fish extended toxicity 14d test, snail survival test. Results The microbiocidal formulation NBF had acute oral toxicity LD50> 5000mg / kg · bw, which belonged to the actual non-toxic substances. The rat acute dermal toxicity LD50> 2000mg / kg · bw, Micronucleus test of the polychromatic erythrocytes The micronucleus rate of the bactericidal preparation in each dose group was not significantly different from that of the negative control group (P> 0.05); for the multiple intact skin irritation reaction scores of the rabbits were 0, acute ocular stimulation score Index of 0, are non-irritating; Guinea pig skin allergy test animals in the experimental group and the negative control group of animals no visible difference in the experimental group skin sensitization reaction score 0, no sensitization; fish extended toxicity 14d test And snail survival test were normal. Conclusion Under the experimental conditions, no obvious toxic effect of the bactericidal agent NBF was found.