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目的探讨群体感应分子法尼醇(Farnesol,FOH)联合钙调神经磷酸酶抑制剂环孢菌素A(Cyclosporin A,CSA)对白念珠菌生物膜形成、形态转换、生长与活性相关基因表达水平的影响,并对相关作用机制进行研究。方法体外构建生物膜状态白念珠菌;以未加药为对照组,CSA、FOH、FOH联合CSA为处理组,经不同处理后,利用实时荧光定量PCR(qRT-PCR)法检测白念珠菌在生物膜形成过程中相关基因在mRNA表达水平的变化。结果 62株白念珠菌均具有不同程度的产膜能力。经过不同药物组处理后,白念珠菌生物膜形成的相关基因中,除了转录抑制因子TUP1基因的mRNA表达量上调,其它基因:ALS3、BCR1、HWP1、TEC1、Ume6、CPH1、GAT2、CEK1、CSH1的mRNA表达量与对照组相比均显示不同程度降低,差异具有统计学意义(P<0.05)。结论群体感应分子Farnesol联合钙调神经磷酸酶抑制剂CSA较CSA与Farnesol单一用药在白念珠菌生物膜形成过程中能够更有效的抑制其生物被膜的形成,从而增加其对抗真菌药物的敏感性。
Objective To investigate the effect of the combination of Farnesol (FOH) and calcineurin inhibitor Cyclosporin A (CSA) on the biofilm formation, morphogenesis, growth and activity of Candida albicans Influence, and study the related mechanism. Methods Candida albicans was constructed biofilm in vitro. The untreated group was treated with CSA, FOH and FOH combined with CSA. After different treatment, Candida albicans was detected by real-time quantitative PCR (qRT-PCR) Biofilm formation related genes in the mRNA expression level changes. Results 62 strains of Candida albicans have different degrees of film-forming ability. After treatment with different drugs, the expression levels of TUP1 gene were increased in the Candida albicans biofilm formation genes. Other genes were ALS3, BCR1, HWP1, TEC1, Ume6, CPH1, GAT2, CEK1, CSH1 Compared with the control group, the mRNA expression levels of all the three groups showed different degrees of reduction, the difference was statistically significant (P <0.05). Conclusion Farnesol combined with calcineurin inhibitor CSA can inhibit the formation of biofilm more effectively than monosial of CSA and Farnesol during the formation of C. albicans biofilm and thus increase its sensitivity to antifungal drugs.