论文部分内容阅读
提取方法:将葛根水浸羔1公斤,溶解于1升水(10℃以下)中,用玻璃纸(No.400)包上,置于5升冷水中,一边通入气泡,一边搅拌,约100小时,取透析液在蒸发器上减压浓缩至约1升,加入500克活性炭,在70℃下搅拌2小时,取炭末水洗后,用5升甲醇加热提取,蒸去甲醇,得黄色粉未50克,将其通过氧化铝柱进行层析,用正丙醇-水=1:1洗脱,收集500毫升洗脱液,从最先洗脱的部分得到10克黄色粘稠的液状活性物质,易溶于水、乙醇、甲醇、正丁醇而难溶于苯、氯仿,且含有氮,对碘化铋钾、碘化汞钾、碘-碘化钾等试剂呈阳性反应,被确定为植物性生物碱,称为卡塞因 R/R,下简称
Extraction method: The Pueraria water lamb litter 1 kg, dissolved in 1 liter of water (10 ℃ below), with cellophane (No. 400) wrapped, placed in 5 liters of cold water, while bubbling while stirring, about 100 hours , The dialysate was concentrated on the evaporator under reduced pressure to about 1 liter, 500 g of activated carbon was added and stirred at 70 ° C for 2 hours. After charcoal was washed with water, the mixture was heated and extracted with 5 liters of methanol, and the methanol was distilled off to obtain yellow powder 50 g, which was chromatographed on an alumina column eluting with n-propanol-water = 1: 1. 500 ml of eluate was collected and 10 g of a yellow viscous liquid active substance was obtained from the first eluted fraction , Soluble in water, ethanol, methanol, butanol and insoluble in benzene, chloroform, and contains nitrogen, bismuth potassium iodide, potassium iodide, potassium iodide - potassium iodide and other reagents were positive reaction was identified as botanical Alkaloids, called Casseline R / R, under the abbreviation