论文部分内容阅读
目的:提高复制型HBV转基因小鼠的遗传稳定性。方法:应用回交传代及双杂交育种法,经荧光定量PCR、ELISA和化学发光法研究HBV基因在小鼠体内的复制与表达。结果:HBV转基因小鼠已稳定传至第23代,血清HBsAg达4122.31±2044.74 IU/ml,93.93%的转基因小鼠血清HBV DNA达104~106copies/ml,表达复制水平较早期有显著提高并稳定传代;雌雄小鼠之间表达水平无显著性差异。结论:该转基因小鼠经过培育传代,已成为一个高表达且遗传稳定的复制型HBV小鼠模型。
Objective: To improve the genetic stability of replicative HBV transgenic mice. Methods: The replication and expression of HBV gene in mice were studied by fluorescence quantitative PCR, ELISA and chemiluminescence using backcross generation and two-hybrid breeding. Results: The HBV transgenic mice were stably passaged to the 23rd generation, the serum HBsAg reached 4122.31 ± 2044.74 IU / ml, and the serum HBV DNA of 93.93% of the transgenic mice reached 104-106 copies / ml. The level of replication was significantly increased and stabilized There was no significant difference in the expression level between male and female mice. Conclusion: The transgenic mice have been passaged and have become a highly expressed and genetically stable replicative HBV mouse model.