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在温室条件下,利用PCR-DGGE技术和Q-PCR技术分析了两种转Cry1Ac基因抗虫棉及其亲本棉花在不同取样时期的根际真菌多样性和生物量的动态变化情况,以评价其生态安全.结果表明,转Bt基因棉与对照棉根际真菌优势条带组成在不同的取样时期极为相似,三者间差异条带多为不清晰的条带.转Bt棉SGK321根际真菌的生物量在苗期明显低于亲本对照,转Bt棉XP188根际真菌的生物量在花铃期较对照有轻微降低,其它时期两种转Bt棉根际真菌生物量没有显著降低,甚至还有增加的趋势.不过根际真菌群落结构的改变和生物量的差异不仅存在于转基因棉和其亲本对照棉之间,它同样存在于对照棉SHIYUAN321和JM20之间以及转基因棉SGK321和XP188之间.可见Bt并不是造成转基因棉和非转基因棉根际真菌群落结构差异、多样性改变和生物量减少的唯一诱因,品种的不同也对它们也有影响.
The dynamic changes of rhizosphere fungi diversity and biomass in two cotton cultivars Cry1Ac transgenic cotton and their parents were analyzed by PCR-DGGE and Q-PCR in greenhouse to evaluate their dynamic changes Ecological security.The results showed that the composition of dominant fungal bands in rhizosphere fungi of transgenic Bt cotton and control cotton were very similar at different sampling periods and the difference bands were mostly unclear bands.While transgenic Bt cotton SGK321 rhizosphere fungi At the seedling stage, the biomass was significantly lower than that of the parental control. The biomass of rhizosphere fungi of transgenic Bt cotton XP188 was slightly lower than that of the control at the flowering and boll stage. The fungal biomass of two rhizosphere Bt cotton plants did not decrease significantly at other times However, the change of fungal community structure and biomass in rhizosphere soil was not only found between transgenic cotton and its parental control cotton, but also between control cotton SHIYUAN321 and JM20 and between transgenic cotton SGK321 and XP188. It can be seen that Bt is not the only cause of the difference in rhizosphere fungal community structure, diversity and biomass of transgenic cotton and non-transgenic cotton, and the differences of varieties also affect them.