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选用３０只２８０～３２０ｇ雄性Ｗｉｓｔａｒ大鼠，术前禁食过夜。实验分在体和离体两部分。１在体部分：麻醉动物，开腹，分离肠系膜上动脉（ＳＭＡ），静注肝素６００Ｕ／ｋｇ，观察反复３次２ｍｉｎ、５ｍｉｎ、８ｍｉｎ的缺血和１０ｍｉｎ再灌流制造的预处置，对随后３０ｍｉｎ的缺血和６０ｍｉｎ再灌引起的大鼠小肠损伤的保护作用。２离体部分：分离ＳＭＡ，插管。将肠系膜上静脉（ＳＭＶ）也分离出来。ＳＭＡ插管，连接灌流装置，ＳＭＶ插管收集流出液，用Ｋｒｅｂ－Ｈｅｎｓｅｌｅｉｔ（ＫＨ）明胶液恒温（３７℃）、恒压（１２ｋＰａ）灌流１０ｍｉｎ。用反复３次８ｍｉｎ的停灌和１０ｍｉｎ的复灌制备离体大鼠小肠预处置模型。结果表明，反复３次８ｍｉｎ缺血和１０ｍｉｎ的再灌对小肠损伤的保护作用最明显。预处置在体和离体模型的建立都比较成功
30 male Wistar rats weighing 280-320g were selected and fasted overnight before surgery. Experimental points in vitro and in vivo in two parts. In the body part, animals were anesthetized, the superior mesenteric artery (SMA) was dissected, the heparin 600U / kg was intravenously injected, and the preconditioning was performed after 3min, 5min, 8min ischemia and 10min reperfusion, 30min ischemia and 60min reperfusion induced injury of small intestine in rats. 2 Ex vivo: SMA separation, intubation. The superior mesenteric vein (SMV) is also isolated. The SMA cannula was connected to a perfusion device. The SMV cannula was used to collect the effluent. The Krebs-Henseleit (KH) gelatin solution was incubated at 37 ° C under constant pressure (12 kPa) for 10 min. The isolated rat small intestine preconditioning model was prepared by repeated 3 times of 8 min stop irrigation and 10 min reperfusion. The results showed that three times 8min ischemia and 10min reperfusion on the protective effect of intestinal injury most obvious. Pretreatment in vivo and in vitro models are more successful