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【目的】蚜虫是杂食性农业害虫。本研究旨在通过线粒体基因组分析更好地了解蚜科昆虫的系统发育关系。【方法】结合第二代测序和PCR扩增技术获得了烟蚜Myzus persicae线粒体基因组全序列,与蚜科其他昆虫进行了对比分析;以贝叶斯法和最大似然法基于13个蛋白编码基因对蚜科进行了系统发育分析。【结果】烟蚜线粒体基因组(Gen Bank登录号:KU_236024)序列全长17 832 bp,A+T含量84.1%,AT偏斜为0.094,GC偏斜为-0.296。包含13个蛋白编码基因(cox1-3,nad1-6,nad4L,atp6,atp8和cytb),22个tRNA,2个rRNA基因(rrn L和rrn S)和2个长的非编码区,其基因排列顺序与已知的蚜科昆虫相似,除了nad4以单独的T结尾,所有的蛋白编码基因均以ATN作为起始密码子,TAA作为终止密码子。在烟蚜线粒体基因组中,tRNAGlu和tRNAPhe中间有一段307 bp的非编码区,该编码区包含2个重复单元,烟蚜的控制区长2 531 bp,是所有测序蚜虫线粒体基因组中最长的。rrn L的二级结构包含6个结构域,44个茎环结构;rrn S的二级结构有3个结构域,24个茎环结构。基于烟粉虱和其他20种昆虫的13个蛋白编码基因重建的BI和ML系统发育树,与传统形态学分类结果一致。【结论】蚜亚科和长管蚜亚科的单系性得到了很好的支持;在长管蚜亚科的分支中,M.persicae与D.noxia聚成一支,并且C.salicicola位于进化枝的底部。本研究结果为蚜科系统发生关系重建积累了有价值的数据资料。
【Aim】 Aphids are omnivorous agricultural pests. This study aims to better understand the phylogenetic relationships of aphididae through mitochondrial genome analysis. 【Method】 The full-length mitochondrial genome of Myzus persicae was obtained by combining the second-generation sequencing and PCR amplification. The sequences were compared with other Aphididae insects. Bayesian method and maximum likelihood method were based on 13 protein-coding genes Aphid family phylogenetic analysis. 【Result】 The sequence of mitochondrial genome of Myzus persicae (GenBank accession number: KU_236024) was 17 832 bp in length with A + T content of 84.1%, AT deviation of 0.094 and GC bias of -0.296. Contains 13 protein-coding genes (cox1-3, nad1-6, nad4L, atp6, atp8 and cytb), 22 tRNAs, 2 rRNA genes (rrn L and rrn S) and 2 long non- The order is similar to the known Aphididae except that nad4 ends with T alone, with all protein-coding genes using ATN as the start codon and TAA as the stop codon. In the mitochondrial genome of Myzus persicae, there is a 307 bp non-coding region between tRNAGlu and tRNAPhe. The coding region contains 2 repeats. The control region of Myzus persicae is 2 531 bp, which is the longest among all sequenced aphids mitochondrial genome. The secondary structure of rrn L contains six domains and 44 stem-loop structures; the secondary structure of rrn S has three domains and 24 stem-loop structures. The BI and ML phylogenetic trees based on 13 protein-coding genes of B. tabaci and other 20 insects were consistent with the results of traditional morphological classification. 【Conclusion】 The monophyletic families of Aphididae and Aphididae have been well supported. In the branch of Aphididae, M.persicae and D.noxia are clustered together, and C.salicicola is located in the area of evolution The bottom of the branch. The results of this study have accumulated valuable data for the phylogenetic relationship of Aphididae.