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目的 探讨广西HCV高危人群庚型肝炎病毒 (HGV)的感染情况及其新基因型的核苷酸序列。方法 静脉药瘾者 (IVDAs) 85份、肝病患者 (PLDs) 80份和献血员 (BDs) 5 0份血清标本 ,用PCR法检测庚型肝炎病毒RNA ,EIA法检测HBsAg、抗 HCV和抗 HIV ;随机选出 6 2份庚型肝炎病毒RNA阳性标本进行核苷酸序列分析 ,构建种系发生树作基因分型。结果 2 15份血清中HGV阳性者85份 (39 5 3% ) ,HBsAg、抗 HGV和抗 HIV的阳性率分别为 39 0 7%、4 2 79%和 0 ;11份HGVRNA的测序结果证实其有 3种基因型 ,其中 5株为新基因型 (亚洲型 ) ,5 1份补测序 ,其中GBV C型占 3 2 3% ,HGV型占 30 6 5 % ,亚洲型占 6 4 5 1%。结论 HGV的 3种基因型中存在不同的基因亚型 ;广西IVDAs、PLDs和BDs中感染庚型肝炎病毒以亚洲型和HGV型为主。
Objective To investigate the infection of hepatitis G virus (HGV) and the nucleotide sequence of its new genotype in Guangxi high-risk HCV population. Methods 85 patients with IVDAs, 80 PLDs and 50 blood donors were enrolled in this study. Hepatitis G virus RNA was detected by PCR, HBsAg, anti-HCV and anti-HIV were detected by EIA ; Randomly selected 6 2 positive samples of hepatitis G virus RNA nucleotide sequence analysis, construction of phylogenetic tree for genotyping. Results The positive rates of HBsAg, anti-HGV and anti-HIV were 85.7%, 42.79% and 85%, respectively. The sequencing results of 11 HGV RNAs showed that There are 3 genotypes, of which 5 are new genotypes (Asian) and 51 are complements, of which GBV type C accounted for 32.3%, HGV type accounted for 30 65%, Asian type accounted for 54 55% . Conclusion There are different genotypes in the three genotypes of HGV. In Guangxi IVDAs, PLDs and BDs, hepatitis G virus is predominant in Asia and HGV.