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目的探讨HOG 1基因在小鼠系统性白念珠菌感染中的作用。方法建立小鼠系统性白念珠菌感染动物模型,设置实验组,HOG 21组(hog1/hog1双等位基因缺陷株);对照组,CAF-2组(标准株)。进行死亡率观察并描绘生存曲线;平皿稀释法检测脾肾组织菌落形成单位(CFU)数目;PAS染色法评估肾脏组织病理学改变。结果实验组小鼠死亡率为0;对照组小鼠死亡率100%;感染后第1~3天,实验组小鼠脾肾带菌量CFU值明显低于对照组(P<0.05),差异有统计学意义;感染后第3天和第5天,实验组小鼠肾脏组织病理检查见小脓肿和微小脓肿,偶见菌丝和孢子;对照组小鼠见大量的菌丝和孢子在肾髓质肾乳头交织成团,伴有大量的中性粒细胞浸润。结论 HOG 1基因在小鼠系统性白念珠菌感染的动物模型中发挥一定的致病性作用。
Objective To investigate the role of HOG 1 gene in systemic Candida albicans infection in mice. Methods The animal model of Candida albicans infection in mice was established. Experimental group, HOG 21 group (hog1 / hog1 double allele deficient strain), control group and CAF-2 group (standard strain) were established. The death rate was observed and the survival curve was drawn. The numbers of colony forming units (CFU) in spleen and kidney were detected by plate dilution method. Pathological changes of renal tissues were evaluated by PAS staining. Results The death rate of mice in the experimental group was 0; in the control group, the mortality of mice was 100%; in the 1st to 3rd days after infection, the CFU of spleen and kidney in experimental group was significantly lower than that of the control group (P <0.05) Statistical significance; 3 days and 5 days after infection, the experimental group of mice kidney tissue pathology showed small abscesses and small abscesses, occasionally mycelium and spores; control group mice to see a large number of mycelium and spores in the kidney Kidney nipple intertwined into groups, accompanied by a large number of neutrophil infiltration. Conclusion HOG 1 gene has certain pathogenicity in animal model of Candida albicans infection in mice.