论文部分内容阅读
目的肿瘤放射敏感性是影响肿瘤放射治疗效果的重要原因。在众多影响放射敏感性的因素中,细胞周期、细胞凋亡和DNA损伤修复发挥重要作用。E2F基因家族通过编码E2Fs转录因子家族,调控细胞周期、细胞凋亡等生命活动。本研究旨在总结E2F基因与肿瘤细胞放射敏感性的关系的研究进展。方法应用PubMed、CNKI等数据库,以“E2F,放疗敏感性,辐射敏感性”等为关键词,检索1990-12-2017-05的中英文文献,共检索到英文文献1 560篇,中文文献634篇。纳入标准:(1)E2F的结构其对细胞周期,细胞凋亡,DNA损伤调控的相关研究;(2)肿瘤细胞辐射敏感性影响因素的临床、基础研究;(3)E2F基因与肿瘤放射敏感性关系的基础研究。排除标准:(1)讨论与辐射敏感性因素关系不紧密的研究;(2)探究不与E2F基因相互作用的基因和细胞因子的研究。符合分析的文献126篇,72篇纳入分析。结果E2F基因通过PRb/E2F通路调控G1/S期转变,进而调控细胞周期进程。细胞DNA损伤后,E2F1通过p53依赖型和非p53依赖型方式诱导细胞凋亡,成为肿瘤放射治疗的潜在靶点。结论探究E2F基因与肿瘤放射敏感性的关系将可能成为未来提高肿瘤放射敏感性的重要思路。
The purpose of tumor radiosensitivity is an important factor that affects the radiotherapy effect of tumors. Among the many factors that affect radiosensitivity, cell cycle, apoptosis and DNA damage repair play an important role. E2F gene family through encoding E2Fs transcription factor family, regulating cell cycle, apoptosis and other life activities. The purpose of this study is to summarize the research progress of the relationship between E2F gene and radiosensitivity of tumor cells. Methods The databases of PubMed and CNKI were used to search the Chinese and English documents of 1990-12-2017-05 with the key words of “E2F, radiosensitivity and radiosensitivity”. A total of 1 560 articles were retrieved from English, 634 articles. Inclusion criteria: (1) The structure of E2F is related to the regulation of cell cycle, apoptosis and DNA damage; (2) Clinical and basic research on the factors affecting the radiosensitivity of tumor cells; (3) The relationship between E2F gene and tumor radiosensitivity Basic research on sexual relations. Exclusion criteria: (1) To discuss studies that are not closely related to radiation-sensitive factors; and (2) to explore the studies of genes and cytokines that do not interact with the E2F gene. 126 articles conforming to the analysis and 72 articles were included in the analysis. Results The E2F gene regulated the G1 / S phase through the PRb / E2F pathway and then regulated the cell cycle progression. After DNA damage, E2F1 induces apoptosis in p53-dependent and non-p53-dependent manner, and becomes a potential target of tumor radiotherapy. Conclusion To explore the relationship between E2F gene and tumor radiosensitivity may be an important way to improve the radiosensitivity of tumors in the future.