Molecular hydrogen regulates the expression of miR-9,miR-21 and miR-199 in LPS-activated retinal mic

• 作 者: Guo-Dan Liu Hong Zhang Lin Wang Qing Han Shi-Feng Zhou Ping Liu
• 机 构: Department of Ophthalmology, the Fourth Affiliated Hospital of Harbin Medical University,Department
• 出 处: International Journal of Ophthalmology(English Edition)
• 发表日期: 2013年03期
• 关键词: microglia protective saturated signaling modulating scavenging miRNAs explain in

AIM: To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities. ·METHODS: Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen -saturated medium or normal medium without hydrogen. qRT -PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS -induced signaling proteins, including Myd88, IKK -β, NF -kB, and PDCD4, were detected by Western blotting. ·RESULTS: The results demonstrated a marked down- regulation of miR -9 and miR -21 and up -regulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-β was decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-kB expression. · CONCLUSION: The results in the present study indicate that miR -9, miR -199 and miR -21 play an important role in the anti -inflammatory regulation of LPS -activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury.

AIM: To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities. · METHODS: Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. QRT -PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Furthermore, the expression of LPS-signaling signaling proteins, including Myd88, IKK-beta, NF- , were detected by Western blotting. RESULTS: The results demonstrated marked marked regulation of miR-9 and miR-21 and upregulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-beta was decreased after hydrogen The results in the present study indicate that miR-9, miR-199 and miR-21 play an important role in the anti-inflamatory regulation of L PS -activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury.