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叶片是水稻主要的光合器官,适度卷曲有利于保持植株叶片直立而不披垂,增加中、下层叶片透光率,从而改善群体光照条件,是理想株型的重要组成,对水稻高产育种具有重要意义.利用甲基黄酸乙酯(EMS)诱变籼稻恢复系缙恢10号获得了一个遗传稳定的水稻生育后期卷叶突变体lrl1.lrl1的叶片在前期生长正常,从13叶龄开始,上三叶沿中脉向内卷曲,且随着生育期推进,卷曲度增加,在成熟期剑叶、倒二叶和倒三叶的卷曲度分别为73.66%,66.91%和45.81%.与野生型缙恢10号相比,除lrl1的千粒重(21.43 g)显著降低外,其他重要农艺性状均没有显著差异.lrl1的叶片小维管束间的泡状细胞数量减少、形状怪异、排列极不规则,导致小维管束之间的夹角变小,从而引起了其叶片的卷曲.lrl1的上三叶光合色素含量均显著高于野生型.但其功能叶净光合速率等均与野生型没有显著差异.经遗传分析和分子定位,该叶片卷曲受一对隐性核基因控制,位于第9染色体分子标记SWU-1和Ind6之间812 kb的区域.通过基因预测,在该区域共有129个候选基因,对其中3个可能与卷叶相关的基因测序,均未发现它们在lrl1与野生型间存在差异.以泡状细胞变化相关的6个卷叶基因在突变体lrl1中的real-time PCR分析表明,卷叶基因ROC5和RL14的表达明显上调,而ACL1,SRL1以及NAL7被下调,暗示了这些基因可能在同一通路上调控叶片的发育.该基因是一个新发现的基因,而且遗传行为简单,其相应突变体含有许多育种有利的性状,因而研究结果为该基因的克隆和功能研究及高产育种奠定了良好基础.
Leaf is the main photosynthetic organ in rice. The moderate curl helps to keep plant leaves upright without drape, and increases light transmittance of middle and lower leaves to improve population illumination conditions. It is an important component of ideal plant type and important to high-yielding rice The results showed that the leaves of lrl1.lrl1, a late leaf-opening mutant of rice, had a stable growth in the early stage, which was inherited from the indica rice restorer line Jinhui 10 by methyl ethylphosphate (EMS) The curlyness of the upper three leaves curled inward along the midrib, and the curl increased with the growth of the three leaves at the mature stage, and the curl degrees of flag leaf, inverted leaf and inverted leaf were 73.66%, 66.91% and 45.81% Compared with Jinhui 10, no significant difference was found in other important agronomic traits, except that the 1000-grain weight (21.43 g) of lrl1 was significantly reduced.The number of vesicular cells among the small vascular bundles of the leaves of lrl1 decreased, the shapes were weird and the arrangement was extremely irregular, Resulting in the small angle between the small vascular bundles, which led to the curling of its leaves.rl1 the upper three-leaf photosynthetic pigment content were significantly higher than the wild-type.However, the functional leaf net photosynthetic rate Dengjun no significant difference with the wild type Through genetic analysis Which is controlled by a pair of recessive nuclear genes and located in the region of 812 kb between molecular markers SWU-1 and Ind6 on chromosome 9. By gene prediction, there are 129 candidate genes in this region, of which 3 There was no difference between lrl1 and wild type in the gene sequences probably related to leaf curl.Real-time PCR analysis of 6 leaf-roll genes related to vesicular cell change in the mutant lrl1 showed that the leaf-opening gene ROC5 and RL14 were significantly up-regulated, while ACL1, SRL1 and NAL7 were down-regulated, suggesting that these genes may regulate leaf development in the same pathway.This gene is a newly discovered gene with simple genetic behavior and its corresponding mutant contains Many breeding favorable traits, so the research results for the cloning and function of the gene and laid a good foundation for breeding.