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目的：观察I型钠氢交换蛋白（NHE1）通过调控胃癌细胞SGC-7901内酸碱值（pH）对其迁移、侵袭的影响。方法采用共聚焦显微镜观察比较用特异性阻断剂5-（N-乙基- N-异丙基）阿米洛利（EIPA）阻断胃癌细胞SGC-7901的NHE1功能前后胞内pH值的变化，划痕实验观察细胞迁移能力的改变，transwell侵袭实验观察细胞侵袭能力的改变。结果共聚焦实验发现对照组细胞内pHi为（7.2±0.12），EIPA阻断剂组（5μm）为（7.01±0.23），EIPA阻断剂组细胞内的pH值明显降低（<0.05）；进一步划痕和侵袭实验证明加入不同浓度的EIPA （5、10和20μmol）后均能有效地抑制胃癌细胞SGC-7901的迁移和侵袭能力（<0.05，<0.01），并且抑制的程度与EIPA的浓度呈剂量依赖性。结论阻断NHE1可以降低胃癌细胞pH值，进而抑制胃癌细胞的迁移和侵袭能力。“,”objective To observe the effect of Na+/H+ exchanger 1 (NHE1)-regulated intracellular pH on the invasion and migration of gastric cancer cell line SGC-7901. Methods The gastric cancer cell line SGC -7901 was treated with the NHE1 specific inhibitor 5-(N-ethyl-N-isopropyl) Amiloride (EIPA), the intracellular pH of SGC-7901 was measured using confocal fluorescence microscope. The migration of SGC-7901 cells was examined by scratch test. The invasion of SGC-7901 cells was examined by transwell chamber invasive test. Results The intracellular pH in the control group was (7.2 ± 0.12), it significantly reduced to (7.01 ± 0.23) in the 5-μmol EIPA group. EIPA of different concentrations (5 μmol, 10 μmol and 20 μmol) could inhibit the invasion and migration of SGC-7901 cells in a dose-dependent manner. Conclusions NHE1 plays an important role in the regulation of intracellular pH. Blockage of NHE1 could restrain the invasion and migration of gastric cancer cells.