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应用 RAPD和 AFL P的 DNA指纹图谱方法分析 2 5个甜 (辣 )椒和 2 2个番茄品种的真实性 ,并进一步比较RAPD和 AFL P的 DNA指纹图谱在鉴定亲缘关系较近的品种之间的真实性时的有效性。对于甜 (辣 )椒品种 ,AFL P方法中 ,2个引物组合扩增反应的多态性片段即能将 2 5个品种完全分开。虽然 ,每个样品的 AFL P的扩增产物中多态性片段的百分率为 9% ,低于 RAPD的 35 %多态性片段的百分率。但是 ,AFL P的信息量远远大于RAPD的信息量 ,它的每对引物组合扩增的平均多态标记为 5 .1,而 RAPD仅为 2 .2。所以 ,在甜 (辣 )椒的指纹图谱中 ,AFL P的有效率是 RAPD的 2倍。对于番茄品种 ,每个样品的 AFL P的扩增产物中多态性片段的百分率为5 .5 % ,大大低于 RAPD的单引物 6 1%多态性片段和双引物 5 8%多态性片段的百分率。它的每对引物组合扩增反应的平均多态标记为 2 .6 ,而 RAPD中 ,单引物扩增的平均多态标记为 4 .2 ,双引物扩增的平均多态标记为 4 .4。一个单引物和一个双引物的 RAPD扩增反应的多态性片段即能将 2 2个番茄品种中的 2 1个完全分开。所以 ,在番茄的指纹图谱中 ,RAPD的有效率是 AFL P的 2倍。因此 ,在应用分子标记辅助鉴定品种的真实性时 ,不同的作物所适用的方法是不同的。
The DNA fingerprinting of RAPD and AFL P was used to analyze the authenticity of 25 sweet pepper and 22 tomato varieties. The DNA fingerprinting of RAPD and AFL P were further compared to identify the more closely related species The validity of the authenticity. For the sweet pepper varieties, AFL P method, two primer combinations amplified polymorphic fragments that can be 25 species completely separated. Although the percentage of polymorphic fragments in the amplified product of AFL P per sample was 9%, it was lower than the percentage of 35% polymorphic fragments of RAPD. However, the amount of information of AFL P is much larger than the amount of information of RAPD. The average polymorphism of each primer pair amplified is 5.1, while RAPD is only 2.2. Therefore, AFL P is twice as effective as RAPD in the fingerprinting of sweet pepper. For tomato cultivars, the percentage of polymorphic fragments in AFL P amplified products was 5.5% for each sample, significantly lower than that of RAPD single-primer 61% polymorphic fragment and double-primer 58% polymorphism The percentage of fragments. The average polymorphism of each pair of primers was 2.6, while that of RAPD was 4.2 for single-primer amplification and 4.4 for double-primer amplification. . Polymorphic fragments of the RAPD amplification reaction of a single primer and a double primer completely separated 21 of 22 tomato cultivars. Therefore, RAPD is twice as efficient as AFL P in tomato fingerprinting. Therefore, the application of molecular markers to assist in the authenticity of varieties, different crops are applied to the method is different.