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目的探讨牛磺酸对大鼠肝缺血再灌注(I/R)后小肠损伤的保护作用。方法将大鼠随机分成假手术组,肝I/R组,牛磺酸预处理+肝I/R组;采用阻断肝动脉、门静脉30min后再灌注的方法,制作肝I/R模型。各组于再灌注3,6,24h分别采血,测定二胺氧化酶(DAO)数值,检测小肠功能;同步切取小肠,测定肠道组织中的SOD及MDA含量,评价肠道自由基损伤程度;切片后行苏木素-伊红(HE)染色,观察病理形态学改变;原位末端标记法(TUNEL)测定细胞凋亡;免疫组化法测定caspase-3表达。结果与假手术组比较,肝I/R组SOD水平明显降低(P<0.05),MDA和DAO水平明显升高(P<0.05),小肠病理损伤严重,凋亡指数明显升高(P<0.05),caspase-3阳性率明显增加(P<0.05)。与I/R组同时间点比较,牛磺酸预处理+I/R组各项指标均明显改善(均P<0.05)。结论牛磺酸对肝I/R后小肠损伤具有保护作用。
Objective To investigate the protective effect of taurine on intestinal injury after hepatic ischemia-reperfusion (I / R) in rats. Methods The rats were randomly divided into sham-operated group, hepatic I / R group and taurine pretreatment + hepatic I / R group. The hepatic I / R model was made by blocking the hepatic artery and portal vein after 30 minutes of reperfusion. The blood samples were taken at 3, 6 and 24 hours after reperfusion. The values of diamine oxidase (DAO) were measured and the function of small intestine was detected. The small intestine was simultaneously excised and the content of SOD and MDA in intestinal tissue was measured to evaluate the degree of intestinal free radical damage. The sections were stained with hematoxylin and eosin (HE), the pathological changes were observed, apoptosis was detected by TUNEL and the expression of caspase-3 was detected by immunohistochemistry. Results Compared with the sham operation group, the level of SOD in liver I / R group was significantly lower (P <0.05), the level of MDA and DAO was significantly increased (P <0.05), the pathological lesion of small intestine was severe and the apoptosis index was significantly increased (P <0.05 ), the positive rate of caspase-3 increased significantly (P <0.05). Compared with I / R group, taurine preconditioning + I / R group improved significantly (all P <0.05). Conclusion Taurine has a protective effect on small intestine injury after hepatic I / R.