论文部分内容阅读
取胼胝600mg,在含6M尿素的Tris-Hcl缓冲液(pH 7.4)中高速粉碎及超声匀浆,高速离心弃去水溶性蛋白,再以0.1Mβ-巯基乙醇裂解二硫键,收集含有角蛋白的上清液,角蛋白获得量为220mg。取新西兰大白兔背部皮内多点注射,免疫血清双扩散效价为1:32。收集各种肿瘤的外检蜡块202例,免疫过氧化物酶法观察其角蛋白分布情况。其中上皮来源101例,间叶各组织36例,神经外胚层24例,双向
Callus 600mg, in 6M urea Tris-Hcl buffer (pH 7.4) high speed grinding and ultrasound homogenate, high speed centrifugation to discard the water-soluble protein, and then 0.1M β-mercaptoethanol disulfide disulfide bond, collecting keratin Of supernatant, the amount of keratin obtained was 220 mg. New Zealand white rabbits were injected intradermally on the back of the dermis, and the double titer of immune serum was 1:32. A total of 202 cases of wax samples were collected from various kinds of tumors, and the distribution of keratin was observed by immunoperoxidase method. 101 cases of epithelial origin, 36 cases of mesenchymal tissue, 24 cases of neuroectoderm, two-way