The Expression of Endothelin Receptor B in Melanoma Cells A375 and Sk-mel-1 and the Proliferative Ef

• 作 者: 林能兴 黄长征 田进 陶娟 张进 杨凌云 李延 刘业强 陈思远 沈关心 李家文 王椿森 涂亚庭
• 机 构: Department of Dermatology Union Hospital Tongji Medical College Huazhong University of Science and
• 出 处: Journal of Huazhong University of Science and Technology(Med
• 发表日期: 2007年05期
• 关键词: Endothelin endothelin melanoma proliferative Receptor manner enhancing assessed

In order to investigate the expression of endothelin receptor B(ETR-B) in human malignant melanoma(MM) cells A375 and SK-mel-1 and the proliferative effects of endothelin 3(ET3) on A375 cells,RT-PCR was applied to detect the expression of ETR-B gene in human MM cells A375 and SK-mel-1.MTT method was used to evaluate the growth enhancing effects of ET3 on A375 cell line in vitro.The results showed that ETR-B gene was expressed in both MM A375 and SK-mel-1 cells.ET3 had stronger ability to enhance the proliferation of A375 cells in vitro in a concentration-dependent manner.It was suggested that ET3/ETR-B might play an important proliferative role in MM.