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本研究设计了向日葵白锈病菌(Albugo tragopogi(Persoon)Schr?ter var.helianthi Novotelnova)的Padlock探针,并对探针的灵敏度和特异性进行了验证。结果表明:该探针特异性强,只能从目标病原菌扩增出102 bp的片段,而在非目标病原菌中不能扩增出该片段,探针的灵敏度可达10 pg/μL。将设计的探针与基因芯片技术相结合,对田间向日葵种子进行检测,实现了对向日葵白锈病菌的高通量检测,本研究建立的检测技术可为口岸和田间开展向日葵其他重要病原菌检测提供参考。
In this study, the Padlock probe of Albugo tragopogi (Persoon) Schröter var. Helianthi Novotelnova was designed and the sensitivity and specificity of the probe were verified. The results showed that the probe was of high specificity and could only amplify a 102 bp fragment from the target pathogen, but could not amplify the fragment in a non-target pathogen. The sensitivity of the probe was 10 pg / μL. The designed probes and gene chip technology combine to detect sunflower seeds in the field and achieve high-throughput detection of sunflower white rust bacteria. The detection technology established in this study can provide the detection for other important pathogenic bacteria of sunflower in ports and fields reference.