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目的:探讨黄芪、小蓟通过NF-κB信号通路对大鼠肾小球系膜细胞的影响。方法:体外培养大鼠肾小球系膜细胞,用血管紧张素Ⅱ刺激系膜细胞增生,分别给予黄芪、小蓟,用酶联免疫吸附(ELISA)法检测肾小球系膜细胞上清液NF-κB和FN的表达。结果:与正常组比较,模型组和黄芪高、低剂量组,小蓟高、低剂量组大鼠肾小球系膜细胞中NF-κB和FN表达均升高且差异有统计学意义(P<0.05);与模型组比较,黄芪高、低剂量组、小蓟高、低剂量组均可降低NF-κB信号转导通路中NF-κB及FN的表达,差异有统计学意义(P<0.05)。结论:黄芪、小蓟可能通过下调肾小球系膜细胞NF-κB和FN蛋白表达的水平,从而调整核因子信号转导通路的兴奋性,减轻肾小球的损伤,减缓肾小球的硬化。
Objective: To investigate the effect of Astragalus membranaceus and Artichoke on NF-κB signaling pathway in rat mesangial cells. Methods: Rat glomerular mesangial cells were cultured in vitro, and mesangial cells were stimulated with angiotensin Ⅱ. Astragalus membranaceus and Artemisia annua were treated with angiotensin Ⅱ, and mesangial cell supernatants were detected by enzyme-linked immunosorbent assay (ELISA) NF-κB and FN expression. Results: Compared with the normal group, the expression of NF-κB and FN in the glomerular mesangial cells of the model group, Astragalus high and low dose groups, Artemisia regia high and low dose groups were increased and the difference was statistically significant (P <0.05). Compared with the model group, the expression of NF-κB and FN in the NF-κB signal transduction pathway was significantly decreased in astragalus high-dose group, low-dose astragalus membranaceus group and high-dose low-dose group, the difference was statistically significant (P < 0.05). Conclusion: Astragalus membranaceus and Artemisia annua could regulate the excitability of NF-κB and FN by regulating the expression of NF-κB and FN in glomerular mesangial cells, reduce the glomerular damage and slow the glomerular sclerosis .