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采用2代Illumina Hi-Seq测序技术对朝仓花椒雌株结果期幼芽的转录组进行测序,构建了转录组数据库,获得49 672 080条Clean Reads数据,包含总长度为4 967 208 000 nt序列数据信息;经拼接组装,获得转录组基因信息长达55 902 243 nt的176 407个Contig片段,再通过进一步拼接,共获得平均长度为878 nt的96 475个Unigene片段。与Nt、Nr、Swiss-Prot、COG、GO、KEGG等数据库进行BLAST信息比对(E-value艽10-5),共获得68 315个注释基因,以及62 150个表达序列标签(EST)。与NR数据库比对,发现花椒转录组基因序列与同科柑橘属的甜橙(Citrus sinensis)和克莱门柚(Citrus clementina)具有较高的同源性,分别为43.96%及41.86%,与其他物种的同源性较低,均不足10%;可将花椒转录组的Unigene的功能通过与COG数据库进行注释比对划分为25类;根据GO数据库的注释,可将其分为生物过程、细胞组分和分子功能3大类共55分支,根据与KEGG数据库的比较分析发现朝仓花椒的转录组数据中含有代谢通路相关基因128类,其中包括多种化合物的代谢途径和次生代谢产物的生物合成途径,其中有较多的次生物质代谢途径,如萜类化合物生物合成途径、黄酮类生物合成代谢途径以及花青素的生物合成途径等。
The transcriptome of shoots from Asparagus officinalis L. was sequenced using two generations of Illumina Hi-Seq sequencing technology. The transcriptome database was constructed and 49 672 080 Clean Reads data were obtained, including a total length of 4967 208 000 nt Data were obtained. A total of 176 407 Contig fragments of 55 902 243 nt in the transcriptome were obtained by splicing, and then 96 475 Unigene fragments with an average length of 878 nt were obtained by further splicing. A total of 68 315 annotated genes and 62 150 expressed sequence tags (ESTs) were obtained by BLAST message alignment (E-value 艽 10-5) with databases such as Nt, Nr, Swiss-Prot, COG, GO and KEGG. Comparing with the NR database, we found that the gene sequence of Zanthoxylum bungeanum transcriptome has a high homology of 43.96% and 41.86% with citrus genus Citrus sinensis and Citrus clementina, respectively The homology of other species is low, which is less than 10%. The function of Unigene can be divided into 25 categories by annotation comparison with COG database. According to the GO database annotation, it can be divided into biological processes, According to the comparison with KEGG database, it was found that the transcriptome data of Asakura chinensis contained 128 metabolic pathway-related genes, including metabolic pathways and secondary metabolites of various compounds Biosynthetic pathways, including more secondary metabolites, such as terpenoid biosynthesis pathway, flavonoid biosynthetic pathway and anthocyanin biosynthesis pathway.