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为进一步研究催产素分子的生理功能及空间结构 ,制备识别其特有表位的单抗。用改良的碳二亚胺二步法交联催产素和牛甲状腺球蛋白 ,用常规方法制备针对催产素分子的单抗 ,测定了抗体的亚类、亚型和相对亲和力 ,并采用竞争ELISA法 ,检测各株细胞分泌的单抗和几种催产素的拟似物的交叉反应程度。结果制备出了 3株分泌高效价抗体的杂交瘤细胞 4G11、 2B11和 3F1,其亚类均为IgG1,亚型均为κ型 ,相对亲和力均较高。 3株中 4G11和 2B11可以特异性地和催产素反应 ,而 3F1与AVP、VAS及ASU有较强的交叉反应。催产素单抗可用于阻断催产素的生物学功能等研究
To further study the physiological function and spatial structure of oxytocin molecules, monoclonal antibodies recognizing their unique epitopes were prepared. The modified carbodiimide two-step method was used to cross-link oxytocin and bovine thyroglobulin. The monoclonal antibodies against oxytocin were prepared by conventional methods. The subclasses, subtypes and relative affinities of the antibodies were determined. By competitive ELISA, The degree of cross-reaction of monoclonal antibodies secreted by each cell line and several oxytocin analogues was examined. Results Three hybridoma cells secreting high titer antibody, 4G11, 2B11 and 3F1, were obtained. The subtypes were all IgG1, and the subtypes were both κ-type with high relative affinity. 3 strains of 4G11 and 2B11 can specifically and oxytocin response, and 3F1 and AVP, VAS and ASU have a strong cross-reaction. Oxytocin monoclonal antibody can be used to block the biological function of oxytocin and other studies