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目的测定脑血管病人血浆血管内皮生长因子(VEGF)水平及其与病情轻重的关系,探讨VEGF对脑缺血再灌注损伤的作用。方法用ELISA法测定脑血管病人的血浆VEGF水平,采用线栓法做鼠大脑中动脉缺血再灌注模型,比较缺血对照组和VEGF处理组梗死体积和脑组织含水量的差异。结果脑梗死及脑出血患者急性期血浆VEGF水平明显升高(P<0.05),脑梗死患者在患病3周后VEGF较急性期还高(P<0.05),而脑出血患者则较急性期下降(P<0.05),VEGF水平与病情轻重成正比(P<0.05),应用VEGF组脑梗死的体积和脑细胞含水量较对照组明显降低(P<0.05)。结论脑缺血诱导VEGF的产生,而VEGF能减小梗死体积,减轻脑水肿。
Objective To determine the level of plasma vascular endothelial growth factor (VEGF) in patients with cerebrovascular disease and its relationship with the severity of illness, and to explore the effect of VEGF on cerebral ischemia-reperfusion injury. Methods Plasma VEGF levels in patients with cerebrovascular disease were determined by ELISA. The middle cerebral artery occlusion (MCAO) model was established by thread occlusion. The differences of infarct volume and brain water content in ischemic control group and VEGF-treated group were compared. Results The levels of plasma VEGF in acute cerebral infarction and intracerebral hemorrhage patients were significantly higher than those in acute phase (P <0.05), and those in acute cerebral infarction were higher than those in acute phase (P <0.05) (P <0.05). The level of VEGF was positively correlated with severity of disease (P <0.05). The volume of cerebral infarction and water content of brain cells in VEGF group were significantly lower than those in control group (P <0.05). Conclusion Cerebral ischemia induces the production of VEGF, while VEGF can reduce infarct volume and reduce brain edema.