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通过受精卵原核显微注射的途径，产生带有可诱导型丙肝病毒非结构蛋白３基因（ｎｏｎｓｔｒｕｃｔｕｒａｌ３ｇｅｎｅ）的重组载体的转基因小鼠。采用ＰＣＲ和基因组Ｓｏｕｔｈｅｒｎｂｌｏｔ杂交方法，在２１只新生鼠中筛选到３只建立者小鼠。其中２只与非转基因小鼠进行交配，分别得到８只和６只Ｆ１代小鼠。进而对其基因组ＤＮＡ进行检测，发现其中４只为转基因阳性个体。这些小鼠的获得，为ｎｓ３转基因品系的建立和ＮＳ３蛋白的生物学作用研究准备了条件。
Transgenic mice with a recombinant vector of inducible hepatitis C virus nonstructural 3 gene were generated by pronuclear microinjection of fertilized eggs. Three founder mice were screened from 21 neonatal rats by PCR and Southern blot hybridization. Two of them were mated with non-transgenic mice, and 8 and 6 F1 mice were obtained respectively. And then its genomic DNA was detected and found that 4 of them were transgenics positive individuals. The availability of these mice prepared the conditions for establishing a ns3 transgenic line and studying the biological role of the NS3 protein.