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目的 :本文离体和整体观察人和大鼠肠内菌对黄山药总皂苷(DX)的代谢作用及整体给予DX后吸收入血的有效成分。方法 :用薄层色谱(TLC)及电喷雾质谱(ESI -MS)法检测粪中DX及其代谢产物。整体给予大鼠灌服DX900mg/kg ,于给药后不同时间采集尿及血清样品 ,用ESI -MS检测吸收入血成分。结果 :DX容易被人和大鼠消化道菌群代谢 ,随着代谢时间的延长 ,出现了各种甾体皂苷的降解产物及终产物薯蓣皂苷元 (Dio)。整体实验表明 ,在大鼠血及尿中均发现分子量为415 3的代谢产物 ,经ESI -MS二级质谱分析 ,上述分子量的化合物为Dio。结论 :DX可被人和大鼠肠内菌代谢 ,DX经口服后Dio被吸收入血。
Objective : The metabolism of total saponin (DX) of D. sinensis (DX) in vitro and in vivo by the observer and rat and the effective components absorbed into the blood after administration of DX as a whole. Methods : DX and its metabolites in feces were detected by thin layer chromatography (TLC) and electrospray ionization mass spectrometry (ESI-MS). Rats were given DX 900mg/kg orally and urine and serum samples were collected at different times after administration. ESI-MS was used to detect the absorbed blood components. RESULTS: DX was easily metabolized by the digestive tract of humans and rats. With the prolongation of metabolic time, various steroidal saponin degradation products and diosgenin (Dio) were found. The overall experiment showed that metabolites with a molecular weight of 415 3 were found in rat blood and urine. The ESI-MS secondary mass spectrometry analysis showed that the above molecular weight compound was Dio. Conclusion: DX can be metabolized by intestinal bacteria in humans and rats, and Dio is absorbed into blood after oral administration of DX.