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目的了解自愿婚检人群中艾滋病的感染状况,为制定该人群中艾滋病的防治策略提供依据。方法选取1 000对自愿参加婚检的结婚登记当事人,采集静脉血,使用酶联免疫法(ELISA)和胶体硒法进行艾滋病抗体检测初筛和复检,蛋白免疫印迹法进行确证实验。结果人类免疫缺陷病毒(HIV)抗体检测初筛和复检ELISA法2例S/CO值均大于19,胶体硒法均显示质控带和测试带,确证实验2例均至少检出gp160、gp120、p66、p55、p51、gp41、p31、p24条带,判定HIV抗体阳性2例,感染率0.1%。流行病学调查显示传播途径均为性传播。结论应在婚检人群中开展艾滋病筛查,加强疾病防治与健康相关知识的宣传教育。
Objective To understand the status of HIV infection among voluntary premarital groups and provide basis for developing AIDS prevention and control strategies in this population. Methods A total of 1 000 couples of marriage registration participants who volunteered for premarital examination were collected. Venous blood was collected. ELISA and colloidal selenium method were used for screening and retesting of HIV antibody. Western blotting was used to confirm the experiment. Results Two cases of S / CO value of HIV antibody screening and retesting ELISA were all greater than 19, both of which showed the control band and the test band, confirming that at least 2 cases of gp160, gp120 , P66, p55, p51, gp41, p31 and p24 were detected in 2 cases with HIV antibody positive rate of 0.1%. Epidemiological studies have shown that all routes of transmission are sexually transmitted. Conclusion AIDS screening should be carried out among premarital people and publicity and education on disease prevention and health-related knowledge should be strengthened.